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I started off learning how to subculture 'Grand Nain' bananas available as sterile cultures in Dr. Richard Litz's Cellular Developmental Botany Lab here at TREC. Under the supervision of Pam Moon, I was able to learn the techniques of micropropagation, and develop the aseptic practices needed for successful multiplication of bananas. I developed this project as means to receive the elective credits I needed for my B.F.A. degree from F.I.U. (Florida International University). While doing my research here at TREC, I posted my results over the internet for Professor David Lee. |
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Since the 'Grand Nain' bananas were already in culture, it was easy to start. First I made 1.5 liters of medium for bananas which consisted of:
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The instruments required for mixing media are beakers graduated cylinders, and micropipettes. I used a hot plate/stirrer with a magnetic bar to mix the media components together. |
